Journal: bioRxiv
Article Title: Synaptic vesicles that store monoamines and glutamate differ in protein composition
doi: 10.1101/2025.05.06.651945
Figure Lengend Snippet: Loss of SCAMP5 impairs synaptic recycling of VGLUT2 SVs without affecting VMAT2 . A . Confocal images and quantification of the fluorescence intensity of synaptotagmin1 and SCAMP5 in uninfected hippocampal neurons or cultures infected with shRNA targeting SCAMP5 or a scrambled control. Each data point represents mean presynaptic SCAMP5 or syt1 intensity per image. Data indicate mean ± SEM, ** p = 0.0021, **** p < 0.0001 by one-way ANOVA with Tukey’s multiple comparisons. n = 5 uninfected, 6 scramble and 7 shRNA coverslips. B-C . Average response curves and quantification of peak fluorescence intensity of VGLUT2-pHluorin (B) or VMAT2-pHluorin (C) to a 60s stimulation at 10 Hz. Data indicate mean ± SEM, * = 0.0225, ** = 0.017 by one-way ANOVA with Tukey’s multiple comparisons. N = 13 scramble, 17 shRNA and 17 rescue coverslips for VGLUT2-pHluorin and 11 scramble, 10 shRNA and 9 rescue coverslips for VMAT2-pHluorin from three independent cultures.
Article Snippet: Hippocampal cultures between 16-19 DIV were mounted in a laminar flow perfusion and stimulation chamber (Warner Instruments RC-21BRFS) on an inverted Nikon Eclipse Ti fluorescence microscope, Hamamatsu ORCA-Flash4.0 camera, and Nikon Plan Apo !D 60x oil objective.
Techniques: Fluorescence, Infection, shRNA, Control